Session 3: Bioprocessing Research

Applied Biochemistry and Biotechnology

, Volume 70, Issue 1, pp 441-462

First online:

Ethanol production from AFEX-treated forages and agricultural residues

  • Khaled BelkacemiAffiliated withDepartment of Food Science and Nutrition, Agri-Food Engineering University Laval
  • , Ginette TurcotteAffiliated withDepartment of Food Science and Nutrition, Agri-Food Engineering University Laval Email author 
  • , Damien de HalleuxAffiliated withDepartment of Soil, Agri-Food Engineering University Laval
  • , Philippe SavoieAffiliated withDepartment of Soil, Agri-Food Engineering University LavalAgriculture and Agri-Food Canada

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Abstract

Lignocellulosic materials derived from forages, namely timothy grass, alfalfa, reed canary grass, and agricultural residues, such as corn stalks and barley straw, were pretreated using ammonia fiber explosion (AFEX) process. The pretreated materials were directly saccharified by cellulolytic enzymes. Sixty to 80% of theoretical yield of sugars were obtained from the pretreated biomasses. Subsequent ethanolic fermentation of the hydrolysates byPachysolen tannophilus ATCC 32691 resulted in 40-60% of theoretical yield after 24 h, based on the sugars present in the hydrolysates. The uptake of sugars was not complete, indicating a possible inhibitory effect onP. tannophilus during the fermentation of these substrates.

Index Entries

Forages agricultural residues AFEX enzymatic hydrolysis ethanolic fermentation biofuel