Journal of Molecular Neuroscience

, Volume 3, Issue 3, pp 137–146

Continuous culture of neuronal cells from adult human olfactory epithelium

  • Benjamin Wolozin
  • Trey Sunderland
  • Bin-bin Zheng
  • James Resau
  • Bernard Dufy
  • Jeffrey Barker
  • Richard Swerdlow
  • Hayden Coon
Article

DOI: 10.1007/BF02919405

Cite this article as:
Wolozin, B., Sunderland, T., Zheng, B. et al. J Mol Neurosci (1992) 3: 137. doi:10.1007/BF02919405

Abstract

Cells from the olfactory epithelium of adult human cadavers have been propagated in primary culture and subsequently cloned. These cells exhibit neuronal properties including: neuron-specific enolase, olfactory marker protein, neurofilaments, and growth-associated protein 43. Simultaneously, the cells exhibit nonneuronal properties such as glial fibrillary acidic protein and keratin, the latter suggesting properties of neuroblasts or stem cells. These clonal cultures contain 5–10% of cells sufficiently differentiated to show odorant-dependent cyclic adenosine 3′,5′-monophosphate (cAMP) or calcium-release responses when challenged with submicromolar concentrations of odorants. The potential of culturing neuronal cells from patients with neuropsychiatric disorders, such as Alzheimer’s disease or schizophrenia, could enable the study of the pathophysiology of these neurons in the culture dish and allow new approaches to the study of mental illness.

Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Benjamin Wolozin
    • 1
  • Trey Sunderland
    • 1
  • Bin-bin Zheng
    • 1
  • James Resau
    • 2
  • Bernard Dufy
    • 3
  • Jeffrey Barker
    • 3
  • Richard Swerdlow
    • 4
  • Hayden Coon
    • 4
  1. 1.Laboratory of Clinical ScienceNIMHBethesdaUSA
  2. 2.Department of PathologyUniversity of Maryland School of MedicineBaltimoreUSA
  3. 3.Laboratory of NeurophysiologyNINDSBethesdaUSA
  4. 4.Division of Cancer Biology and DiagnosisNCIBethesdaUSA