Cell Biophysics

, Volume 4, Issue 2, pp 117–131

Determination of the surface tension of biological cells using the freezing front technique

Authors

  • J. K. Spelt
    • Department of Mechanical EngineeringUniversity of Toronto
  • D. R. Absolom
    • Department of Mechanical EngineeringUniversity of Toronto
    • Research InstituteThe Hospital for Sick Children
    • Department of MicrobiologySUNYAB
  • W. Zingg
    • Research InstituteThe Hospital for Sick Children
    • Institute of Biomedical EngineeringUniversity of Toronto
  • C. J. van Oss
    • Department of MicrobiologySUNYAB
  • A. W. Neumann
    • Department of Mechanical EngineeringUniversity of Toronto
    • Research InstituteThe Hospital for Sick Children
    • Institute of Biomedical EngineeringUniversity of Toronto
Article

DOI: 10.1007/BF02918308

Cite this article as:
Spelt, J.K., Absolom, D.R., Zingg, W. et al. Cell Biophysics (1982) 4: 117. doi:10.1007/BF02918308

Abstract

The freezing front technique for solid surface tension measurements was used to obtain the surface tensions of glutaraldehyde-fixed human erythrocytes, and fresh human lymphocytes and grnulocytes in aqueous media. The results agree well with the values obtained by other methods and indicate that the freezing front technique is sufficiently sensitive to detect small differences (of the order of 0.1 ergs/cm2) in surface tension. This property, along with a number of applications for which it is uniquely suited makes the freezing front technique an important new approach to the measurement of the surface tensions of biological cells and of small particles in general.

Index Entries

Surface tension, of cellsfreezing front technique, for cell surface tensionhuman erythrocytes, surface tension oferythrocytes, human, surface tension oflymphocytes, human, surface tension ofhuman granulocytes, surface tension of

Copyright information

© Humana Press Inc. 1982