Carlsberg Research Communications

, 47:45

Amino acid sequence of serine protease inhibitor CI-1 from barley. Homology with barley inhibitor CI-2, potato inhibitor I, and leech eglin

Authors

  • IB Svendsen
    • Department of ChemistryCarlsberg Laboratory
  • Sigurd Boisen
    • Department of Biochemistry and NutritionTechnical University of Denmark
  • Jørn Hejgaard
    • Department of Biochemistry and NutritionTechnical University of Denmark
Article

DOI: 10.1007/BF02907796

Cite this article as:
Svendsen, I., Boisen, S. & Hejgaard, J. Carlsberg Res. Commun. (1982) 47: 45. doi:10.1007/BF02907796

Abstract

Three molecular forms of a protein inhibitor of chymotrypsin and microbial alkaline proteases have been isolated from Hiproly high-lysine barley.

Automated Edman degradation of one of these inhibitor preparations (CI-1C) resulted in the following amino acid sequence (77 residues in total): Tyr-Pro-Glu-Pro-Thr-Glu-Gly-Ser-Ile-Gly-Ala-Ser-Gly-Ala-Lys-Thr-Ser-Trp-Pro-Glu-Val-Val-Gly-Met-Ser-Ala-Glu-Lys-Ala-Lys-Glu-Ile-Ile-Leu-Arg-Asp-Lys-Pro-Asn-Ala-Gln-Ile-Glu-Val-Ile-Pro-Val-Asp-Ala-Met-Val-Pro-Leu-Asn-Phe-Asn-Pro-Asn-Arg-Val-Phe-Val-Leu-Val (His, Lys, Ala, Thr, Thr, Val, Ala, Glx, Val, Ser, Arg) Val-Gly.

The inhibitor (CI-1) is homologous with another barley inhibitor (CI-2), with potato inhibitor I and with the elastase-cathepsin G inhibitor eglin from the leech Hirudo medicinalis (30–50% of the amino acid residues in identical positions). This established «family of cystine-independent inhibitors» also showed some sequence similarities with the cystine-free yeast proteinase B inhibitors 1 and 2. In the reactive site region homologies with the cystine-rich inhibitors of the «Kazal pancreas secretory inhibitor» and the «Streptomyces subtilisin inhibitor» families were observed.

Keywords

Hiproly barley chymotrypsin inhibitor subtilisin inhibitor

Abbreviations

APNE

N-acetyl-d,l-phenylalanine-2-naphtylester

CI-1 and CI-2

chymotrypsin inhibitor 1 and 2 from barley (3)

LIE

Leech inhibitor eglin (17)

PI-I

potato inhibitor I (15)

Polybrene

hexadimethrine bromide

PTH

phenylthiohydantoin

SDS

sodium dodecyl sulfate

THEED

N,N,N′,N′,-tetrakis (2-hydroxyethyl)ethylenediamine

YIB

yeast inhibitor of protease B (11)

Copyright information

© Carlsberg Laboratory 1982