Carlsberg Research Communications

, 51:509

A non-destructive screening method for proanthocyanidin-free barley mutants

  • Henriette Kristensen
  • Sten Aastrup
Article

DOI: 10.1007/BF02906893

Cite this article as:
Kristensen, H. & Aastrup, S. Carlsberg Res. Commun. (1986) 51: 509. doi:10.1007/BF02906893

Abstract

Barley grains are embedded in a block of plastic clay with the Carlsberg Seed Fixation System, abraded to expose a small sector of endosperm and surrounding testa. Staining of the proanthocyanidins in the testa is carried out by immersion into a solution of vanillin in 4.5 N−HCl. Kernels lacking proanthocyanidins are selected, washed and grown to mature plants. Using sodium azide mutagenesis 10 proanthocyanidin-free mutants with anthocyanin pigmentation in the vegetative parts of the plants were obtained among 78,450 M3-kernels amounting to a frequency of 0.013%.

Keywords

Catechinleucocyanidinanthocyaninvanillin-HCl colour reaction
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Copyright information

© Carlsberg Laboratory 1986

Authors and Affiliations

  • Henriette Kristensen
    • 1
  • Sten Aastrup
    • 2
  1. 1.Department of PhysiologyCarlsberg LaboratoryCopenhagen Valby
  2. 2.Department of Brewing ChemistryCarlsberg Research LaboratoryCopenhagen Valby