Virchows Archiv B

, Volume 63, Issue 1, pp 335–343

Characterization of four new gastric cancer cell lines

Authors

  • H. Peter Vollmers
    • Institut für Pathologie
  • Konrad Stulle
    • Institut für Pathologie
  • Jobst Dämmrich
    • Institut für Pathologie
  • Martin Pfaff
    • Institut für Pathologie
  • Th. Papadopoulos
    • Institut für Pathologie
  • Christoph Betz
    • Institut für Pathologie
  • Katharina Saal
    • Institut für Pathologie
  • Hans-Konrad Müller-Hermelink
    • Institut für Pathologie
Article

DOI: 10.1007/BF02899281

Cite this article as:
Vollmers, H.P., Stulle, K., Dämmrich, J. et al. Virchows Archiv B Cell Pathol (1993) 63: 335. doi:10.1007/BF02899281

Summary

Four well differentiated gastric adenocarcinoma cell lines from German patients have been established from primary tumors (St 23132, St 3051) and lymph node metastases (St 2474, St 2957). The tumor cells were isolated by enzymatic or mechanical treatment. All four lines grew as solid tumors in nude mice and formed colonies in soft agar. The doubling time of the cells in culture was 25–32 h. Further characteristics of the lines were a considerable chromosomal aneuploidy, (the chromosomal numbers varying from 30–109 with many numerical and structural abnormalities), a stable keratin expression (Ck 8, 18, 19), the expression and secretion of CEA and CA-19-9 and the overexpression of c-myc. The four stomach cancer cell lines described here are not only a useful addition to the small number of existing lines, but also represent ideal tools for studying tumorigenicity of human stomach cancers in vitro and in vivo.

Key words

Gastric carcinoma cell linesTumorigenicityKeratin expressionc-myc

Abbreviations

FCS

fetal calf serum

PBS

posphate-buffered saline

SDS

sodium dodecyl sulfate

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Copyright information

© Springer-Verlag 1993