Virchows Archiv B

, 39:173

Ultrastructural study of pancreatic B cell regeneration in newborn rats after destruction by streptozotocin

Authors

  • M. C. Dutrillaux
    • Biologie et Physiologie des Cellules Digestives (U239 Inserm, Dir. F. Potet)Faculté de Médecine X Bichat
  • B. Portha
    • Physiologie du Développement, Laboratoire associé au CNRS 307 (Dir. L. Picon)Université Paris VII
  • C. Rozé
    • Biologie et Physiologie des Cellules Digestives (U239 Inserm, Dir. F. Potet)Faculté de Médecine X Bichat
  • E. Hollande
    • Biologie CellulaireUniversité Paul Sabatier
Article

DOI: 10.1007/BF02892846

Cite this article as:
Dutrillaux, M.C., Portha, B., Rozé, C. et al. Virchows Archiv B Cell Pathol (1982) 39: 173. doi:10.1007/BF02892846

Summary

An ultrastructural study of endocrine cells was performed in the pancreas of rats treated with a single dose of streptozotocin on the day of birth. Most of the B cells present at birth were destroyed by streptozotocin but some survived and could again synthesize insulin after eliminating their altered fragments in phago-lysosome structures. New B cells were produced primarily by the formation of new islets from the small pancreatic ducts. A study of mitosis in colchicine treated animals showed that most B cells present on day 4 were formed by mitosis of undifferentiated cells. No significant division of preexisting differentiated B cells could be shown in streptozotocin treated rats.

B cell regeneration in this newborn rat model is thus explained primarily by budding of new islets from the ducts.

Key words

B cells Streptozotocin Regeneration of endocrine cells Experimental diabetes Endocrine pancreas

Copyright information

© Springer-Verlag 1982