Virchows Archiv B

, 37:125

Immunohistochemical and immunoelectron microscopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph node

  • Kiyoshi Takahashi
  • Haruyasu Yamaguchi
  • Junko Ishizeki
  • Takashi Nakajima
  • Yoichi Nakazato
Article

DOI: 10.1007/BF02892562

Cite this article as:
Takahashi, K., Yamaguchi, H., Ishizeki, J. et al. Virchows Archiv B Cell Pathol (1981) 37: 125. doi:10.1007/BF02892562

Summary

Lymph nodes of 5 cases with non-specific lymphadenitis and of 4 cases with dermatopathic lymphadenitis were examined by the peroxidase anti-peroxidase (PAP) method and by immunoelectron microscopy using monospecific antibody against nervous system specific S-100 protein. S-100 protein was detected in dendritic shaped cells of the thymus-dependent area of non-specific lymphadenitis. The paracortical area of dermatopathic lymphadenitis showed a marked accumulation of S-100 protein-containing dendritic shaped cells. Immunoelectron micrographs revealed that S-100 proteincontaining d endritic shaped cells of both non-specific and dermatopathic lymphadenitis corresponded to interdigitating reticulum cells (IRC). The detection of S-100 protein in the nucleus and on the cytoplasmic and nuclear membrane suggested that IRCs themselves synthesized S-100 protein. S-100 protein is a very useful cell marker for the identification of IRC in the lymphoid tissue.

Key words

S-100 protein Enzyme immunohistochemistry Non-specific lymphadenitis Dermatopathic lymphadenitis Interdigitating reticulum cell 

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Kiyoshi Takahashi
    • 1
  • Haruyasu Yamaguchi
    • 2
  • Junko Ishizeki
    • 1
  • Takashi Nakajima
    • 3
  • Yoichi Nakazato
    • 1
  1. 1.First Department of PathologyGunma University School of MedicineGunma-ken 371Japan
  2. 2.Institute of Neurology and RehabilitationGunma University School of MedicineMaebashiJapan
  3. 3.Pathology DivisionNational Cancer Center Research InstituteTokyo 104Japan
  4. 4.Second Department of PathologyKochi Medical SchoolKochi-kenJapan

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