Journal of Tongji Medical University

, Volume 18, Issue 4, pp 257–261

Biodegradation of tricalcium phosphate ceramics by osteoclasts

  • Zheng Qixin
  • Du Jingyuan
  • Xia Zhidao
  • Zeng Hui
  • Li Shipu
  • Yan Yuhua
  • Chen Fang
Article

DOI: 10.1007/BF02886487

Cite this article as:
Qixin, Z., Jingyuan, D., Zhidao, X. et al. Journal of Tongji Medical University (1998) 18: 257. doi:10.1007/BF02886487

Summary

Biodegradation of tricalcium phosphate (TCP) ceramics was observed through mixed culture of osteoclasts and TCP discsin vitro in this study. Osteoclasts were isolated from newborn SD rat’s marrow of long bone and cultured on TCP discs. The culture terminated at the 48th h and 96th h respectively. Under an inverted microscope, the osteoclasts imparted round or oval body with multinuclear and many thin processes. These cells were positively stained for tartrate-resistance acid phosphatase (TRAP). Scanning electron microscope showed that many resorption lacunae on TCP disc surface and their diameters were smaller than 20 μm. Osteoclasts were located in the lacunae. At the 96th h, the resorption lacunae become larger and osteoclasts showed degeneration. It is suggested that osteoclasts possess ability to re-absorb TCP ceramics underin vitro culturing condition.

Key words

degradationosteoclasttricalcium phosphate

Copyright information

© Springer 1998

Authors and Affiliations

  • Zheng Qixin
    • 1
  • Du Jingyuan
    • 1
  • Xia Zhidao
    • 1
  • Zeng Hui
    • 1
  • Li Shipu
    • 2
  • Yan Yuhua
    • 2
  • Chen Fang
    • 2
  1. 1.Department of Orthopedics, Xiehe HospitalTongji Medical UniversityWuhan
  2. 2.Wuhan Technology UniversityWuhan