Biologia Plantarum

, 38:95

Photophysiology of turion formation and germination inSpirodela polyrhiza

  • K. -J. Appenroth
  • S. Teller
  • M. Horn
Original Paper

DOI: 10.1007/BF02879642

Cite this article as:
Appenroth, K.J., Teller, S. & Horn, M. Biol Plant (1996) 38: 95. doi:10.1007/BF02879642


Standardized laboratory techniques for the vegetative growth of the duckweedSpirodela polyrhiza (Lemnaceaé), and for formation as well as germination of their turions were described. Increasing photon fluence rates of blue or red light increased the yield of turions. A specific stimulating effect of blue light was demonstrated under autotrophic but not under mixotrophic conditions. Therefore the spectral composition of light is not important in mixotrophic formation of turions whereas in autotrophic formation light sources with a higher portion of blue light are recommended. Dark-grown (etiolated) turions showed accelerated germination and higher germination percentage in comparison with light-grown turions after induction by a single red light pulse. This difference was overcome in continuous red light by speeding up the germination response of light-grown turions. Use of Petri dishes (8 cm3 nutrient solution) instead of Erlenmeyer flasks (50 cm3 nutrient solution) retarded germination response. Especially for long term experiments the use of Erlenmeyer flasks is recommended. Storage of turions for 72 h at 25 ‡C following at 5 ‡C in darkness after-ripening resulted in a decreased lag phase of the light-induced germination both after induction by a single light pulse and in continuous light.

Additional key words

blue light receptorduckweedLemnaceaephytochrome





blue light




turions-dark-grown (etiolated) turions


far red light


turions-light-grown turions


red light


“white” light

Copyright information

© Institute of Experimental Botany, ASCR 1996

Authors and Affiliations

  • K. -J. Appenroth
    • 1
  • S. Teller
    • 1
  • M. Horn
    • 1
    • 2
  1. 1.Institute of General BotanyUniversity of JenaJenaGermany
  2. 2.Institute of Medical StatisticsUniversity of JenaJenaGermany