Science in China Series C: Life Sciences

, Volume 40, Issue 1, pp 1–9

Prediction of mutant activity and its application in molecular design of tumor necrosis factor-α

  • Weidong Tang
  • Tao Xi
  • Bo Wang
  • Donglin Guo
  • Xianxiu Xu
  • Dexu Zhu
Article

DOI: 10.1007/BF02879101

Cite this article as:
Tang, W., Xi, T., Wang, B. et al. Sci. China Ser. C.-Life Sci. (1997) 40: 1. doi:10.1007/BF02879101

Abstract

Two models for prediction of the activity and stability of site-directed mutagenesis on tumor necrosis factor-α are established. The models are based on straightforward structural considerations, which do not require the elaboration of sitedirected mutagenesis on the protein core and the hydrophobic surface area by analyzing the pmperties of the mutated amino acid residues. The reliabilities of the models have been tested by analyzing the mutants of tumor necrosis factor-α (TNF-α) whose two leucine residues (L29, L157) were mutated. Based on these models, a TNFα mutant with high activity was created by molecular design.

Keywords

prediction modeltumor neauwia factor-αmolecular design

Copyright information

© Science in China Press 1997

Authors and Affiliations

  • Weidong Tang
    • 1
  • Tao Xi
    • 1
  • Bo Wang
    • 1
  • Donglin Guo
    • 1
  • Xianxiu Xu
    • 1
  • Dexu Zhu
    • 1
  1. 1.Department of Biochemistry and State Key Laboratory of Pharmaceutical BiotechnologyNanjing UniversityNanjingChina