Folia Microbiologica

, Volume 23, Issue 4, pp 286–291

Stimulation of amino acid transport inSaccharomyces cerevisiae by metabolic inhibitors

  • J. Horák
  • A. Kotyk
  • L. Říhová
Article

DOI: 10.1007/BF02876682

Cite this article as:
Horák, J., Kotyk, A. & Říhová, L. Folia Microbiol (1978) 23: 286. doi:10.1007/BF02876682

Abstract

Inhibitors of energy metabolism (3-ohlorophenylhydrazonomalononitrile, antimycin A, iodoacetamide, dicyclohexylcarbodiimide) but not of transport (uranyl ions) stimulate at low concentrations the uptake ofl-leucine,l-glutamic acid,l-argimne and, to a lesser degree, of 2-aminoisobutyric acid inSaccharomyces cerevisiae. The effect is apparent only after augmenting the energy reserves of cells by preincubation withd-glueose or, more strikingly, with ethanol. It is absent in a mutant (op1) lacking the translocation system for ADP-ATP in mitochondria. The presence of two different energy reserves for amino acid transport is indicated (one in energy-poor, the other in energy-rich cells). The stimulating effect appears to be caused by a retarded degradation of the transport proteins as occurs at a lowered level of mitochondria-produced ATP.

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1978

Authors and Affiliations

  • J. Horák
    • 1
  • A. Kotyk
    • 1
  • L. Říhová
    • 1
  1. 1.Laboratory for Cell Membrane Transport, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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