Article

Indian Journal of Clinical Biochemistry

, Volume 18, Issue 2, pp 48-53

First online:

Serodiagnosis of tuberculosis using two ELISA systems

  • Swati BanerjeeAffiliated withJamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical SciencesDepartment of Biochemistry, Mahatma Gandhi Institute of Medical Sciences
  • , Sonika GuptaAffiliated withJamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical SciencesDepartment of Biochemistry, Mahatma Gandhi Institute of Medical Sciences
  • , Niraj ShendeAffiliated withJamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical SciencesDepartment of Biochemistry, Mahatma Gandhi Institute of Medical Sciences
  • , Satish KumarAffiliated withJamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical SciencesDepartment of Biochemistry, Mahatma Gandhi Institute of Medical Sciences
  • , Bhaskar C. HarinathAffiliated withJamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical SciencesDepartment of Biochemistry, Mahatma Gandhi Institute of Medical Sciences Email author 

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Abstract

Serodiagnosis by ELISA has been widely explored over the years, in the diagnosis of tuberculosis. Two ELISA systems were evaluated for detection of mycobacterial antibodies in pulmonary and extra pulmonary tuberculosis. The two test assays explored were ERBA LISA (TB IgG) test (Anda Biologicals) which uses A60 antigen complex found in the cytosol of typical and atypical mycobacteria, and SEVA TB (IgG) ELISA, which uses a 31 kDa, glycoprotein antigen purified fromM. tb H37Ra culture filtrate. Sera from 98 proven tuberculosis [pulmonary TB (48), tuberculous lymphadenopathy (30), tuberculous meningitis (15) & genitourinary TB (5)] were studied along with 32 healthy controls. The overall positivity obtained using ERBA LISA (TB IgG) test and SEVA TB (IgG) ELISA test was 72.9% and 91.6% in pulmonary tuberculosis, 43.3% and 76.6% in tuberculous lymphadenopathy respectively. The sensitivity of ERBA LISA test in tuberculous meningitis and genito-urinary TB was significantly low (26.6% & 40% respectively) compared to sensitivity obtained using SEVA TB ELISA (86.6% & 60% respectively) with overall specificity of 60% and 87.5%. Thus SEVA TB IgG ELISA test was found to be more sensitive than ERBA LISA in detecting IgG antibodies in tuberculous sera, in particular in extra pulmonary tuberculosis cases.

Key Words

ELISA ES-31 serodiagnosis tuberculosis