Indian Journal of Clinical Biochemistry

, Volume 18, Issue 2, pp 48–53

Serodiagnosis of tuberculosis using two ELISA systems

  • Swati Banerjee
  • Sonika Gupta
  • Niraj Shende
  • Satish Kumar
  • Bhaskar C. Harinath
Article

DOI: 10.1007/BF02867367

Cite this article as:
Banerjee, S., Gupta, S., Shende, N. et al. Indian J Clin Biochem (2003) 18: 48. doi:10.1007/BF02867367

Abstract

Serodiagnosis by ELISA has been widely explored over the years, in the diagnosis of tuberculosis. Two ELISA systems were evaluated for detection of mycobacterial antibodies in pulmonary and extra pulmonary tuberculosis. The two test assays explored were ERBA LISA (TB IgG) test (Anda Biologicals) which uses A60 antigen complex found in the cytosol of typical and atypical mycobacteria, and SEVA TB (IgG) ELISA, which uses a 31 kDa, glycoprotein antigen purified fromM. tb H37Ra culture filtrate. Sera from 98 proven tuberculosis [pulmonary TB (48), tuberculous lymphadenopathy (30), tuberculous meningitis (15) & genitourinary TB (5)] were studied along with 32 healthy controls. The overall positivity obtained using ERBA LISA (TB IgG) test and SEVA TB (IgG) ELISA test was 72.9% and 91.6% in pulmonary tuberculosis, 43.3% and 76.6% in tuberculous lymphadenopathy respectively. The sensitivity of ERBA LISA test in tuberculous meningitis and genito-urinary TB was significantly low (26.6% & 40% respectively) compared to sensitivity obtained using SEVA TB ELISA (86.6% & 60% respectively) with overall specificity of 60% and 87.5%. Thus SEVA TB IgG ELISA test was found to be more sensitive than ERBA LISA in detecting IgG antibodies in tuberculous sera, in particular in extra pulmonary tuberculosis cases.

Key Words

ELISAES-31serodiagnosistuberculosis

Copyright information

© Association of Clinical Biochemists of India 2003

Authors and Affiliations

  • Swati Banerjee
    • 1
    • 2
  • Sonika Gupta
    • 1
    • 2
  • Niraj Shende
    • 1
    • 2
  • Satish Kumar
    • 1
    • 2
  • Bhaskar C. Harinath
    • 1
    • 2
  1. 1.Jamnalal Bajaj Tropical Disease Research CentreMahatma Gandhi Institute of Medical SciencesWardhaIndia
  2. 2.Department of BiochemistryMahatma Gandhi Institute of Medical SciencesWardhaIndia