Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 24, Issue 4, pp 385–388

Tetrandrine ameliorates cirrhosis and portal hypertension by inhibiting nitric oxide in cirrhotic rats

Authors

  • Wang Hai
    • The Hepatic Surgery Center, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
  • Chen Xiaoping
    • The Hepatic Surgery Center, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and Technology
Article

DOI: 10.1007/BF02861874

Cite this article as:
Hai, W. & Xiaoping, C. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2004) 24: 385. doi:10.1007/BF02861874

Summary

To examine the role and effect of nitric oxide synthase type II (NOS II) in cirrhotic rats, expression of NOS II mRNA was detected by real time RT-PCR. The enzymatic activity of nitric oxide synthase and the circulating levels of NO, systemic and portal hemodynamics and quantification of cirrhosis were measured. Chinese traditional medicine was used to treat cirrhotic rats and the effect of NO was evaluated. Double-blind method was used in experiment. Our results showed the concentration of NO and the enzymatic activity of NOS increased markedly at all stages of cirrhosis and iNOSmRNA was strongly expressed. Meanwhile, the portal-venous-pressure (PVP) and portal-venous-flow (PVF) were significantly increased. NO, NOS and iNOSmRNA were positively correlated to the degree of hepatic fibrosis. Tetrandrine significantly inhibited NO production and the expression of iNOSmRNA. Our results suggested that increased hepatic expression of NOS II is one of the important factors causing cirrhosis and portal hypertension. Tetrandrine can significantly ameliorate cirrhosis and portal hypertension.

Key words

induced nitric oxide synthasemRNAtetrandrinehepatic cirrhosisportal hemodynamicspathogenesis

Copyright information

© Springer 2004