Fatigue cracking is one of the major failure modes in flexible pavements. Prediction of fatigue life is commonly encountered in the mechanistic-empirical (M-E) flexible pavement design as a transfer function that relates the pavement responses to pavement performance. Thus, development of accurate fatigue cracking prediction models is an ongoing pursuit of the pavement engineering community. This has resulted in a plethora of fatigue prediction models ranging from purely mechanistic to empirical. This paper presents the development of a mechanistic-empirical fatigue cracking prediction model using data from in-service flexible pavements in Michigan, USA. For further validation, three flexible pavement design cases were obtained from the Michigan Department of Transportation (MDOT) and were then compared to the proposed M-E design using the proposed fatigue cracking prediction model as a transfer function.