Plant Molecular Biology Reporter

, Volume 18, Issue 3, pp 243–253

Chinese spring wheat (Triticum aestivum L.) chloroplast genome: Complete sequence and contig clones

  • Yasunari Ogihara
  • Kazuriho Isono
  • Toshio Kojima
  • Akira Endo
  • Mitsumasa Hanaoka
  • Takashi Shiina
  • Toru Terachi
  • Shigeko Utsugi
  • Minoru Murata
  • Naoki Mori
  • Shigeo Takumi
  • Kazuho Ikeo
  • Takashi Gojobori
  • Rika Murai
  • Koji Murai
  • Yoshihiro Matsuoka
  • Yukari Ohnishi
  • Hikaru Tajiri
  • Koichiro Tsunewaki
Genetic Resources

DOI: 10.1007/BF02823995

Cite this article as:
Ogihara, Y., Isono, K., Kojima, T. et al. Plant Mol Biol Rep (2000) 18: 243. doi:10.1007/BF02823995

Abstract

Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat,Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA—a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.

Key Words

Chinese Spring wheatchloroplast DNAclone bankcomplete nucleotide sequenceplastome evolution

Copyright information

© Springer 2000

Authors and Affiliations

  • Yasunari Ogihara
    • 1
  • Kazuriho Isono
    • 2
  • Toshio Kojima
    • 1
  • Akira Endo
    • 1
  • Mitsumasa Hanaoka
    • 3
  • Takashi Shiina
    • 3
  • Toru Terachi
    • 4
  • Shigeko Utsugi
    • 5
  • Minoru Murata
    • 5
  • Naoki Mori
    • 6
  • Shigeo Takumi
    • 6
  • Kazuho Ikeo
    • 7
  • Takashi Gojobori
    • 7
  • Rika Murai
    • 8
  • Koji Murai
    • 8
  • Yoshihiro Matsuoka
    • 8
  • Yukari Ohnishi
    • 8
  • Hikaru Tajiri
    • 8
  • Koichiro Tsunewaki
    • 8
  1. 1.Kihara Institute for Biological ResearchYokohama City UniversityYokohamaJapan
  2. 2.PE Biosystems Japan Ltd.ChibaJapan
  3. 3.Graduate School of Human and Environmental StudiesKyoto UniversityKyotoJapan
  4. 4.Department of Biotechnology, Faculty of EngineeringKyoto Sangyo UniversityKyotoJapan
  5. 5.Research Institute for BioresourcesOkayama UniversityKurashikiJapan
  6. 6.Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of AgricultureKobe UniversityKobeJapan
  7. 7.Center for Information BiologyNational Institute of GeneticsMishimaJapan
  8. 8.Department of BioscienceFukui Prefectural UniversityFukuiJapan