Abstract
Schizosaccharomyces pombe isolated from palm wine was evaluated for single-cell-protein production using hydrocarbon feedstocks. The isolate was adapted for hydrocarbon utilization by continuous enrichment in a mineral-salts medium containing a mixture of two petroleum fractions, kerosene and diesel. The isolate grew well on the mineral-salts medium containing either kerosene or diesel as sole carbon and energy source. Hydrocarbon degradation using the isolate was demonstrated by gas chromatographic analysis. Optimum growth ofS. pombe was obtained at hydrocarbon feedstock concentrations of 1.5%. The optimum temperature and pH for growth of the yeast were found to be 30 °C and 5.5 respectively. Ammonium sulfate was found to be the most suitable nitrogen source. Total protein content of the hydrocarbon-grown yeast, analyzed by the Kjeldahl method, was high. The yeast was capable of synthesizing all the amino acids tested. These results show the feasibility of usingS. pombe grown on hydrocarbon feedstocks to produce single-cell protein.
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Amanchukwu, S.C., Obafemi, A. & Okpokwasili, G.C. Single-cell-protein production bySchizosaccharomyces pombe isolated from palmwine using hydrocarbon feedstocks. Folia Microbiol 34, 112–119 (1989). https://doi.org/10.1007/BF02823688
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DOI: https://doi.org/10.1007/BF02823688