Micropropagation Review

In Vitro Cellular & Developmental Biology - Plant

, Volume 34, Issue 2, pp 122-130

Benefits ofin vitro “biotization” of plant tissue cultures with microbial inoculants

  • Jerzy NowakAffiliated withDepartment of Plant Science, Nova Scotia Agricultural College

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In vitro coculture of plant tissue explants with beneficial microorganisms induces developmental and metabolic changes in the derived plantets which enhance their tolerance to abiotic and biotic stresses. The induced resistance response caused by the inoculants is referred to as “biotization.” There is enough experimental evidence with bacteria (bacterization) and vesicular arbuscular mycorrhiza (mycorrhization) inoculations to recommend utilization of this technology in commercial micropropagation. The paper reviews literature on this topic, outlines other uses and potential uses ofin vitro plant-microbial cocultures, and discusses their current constraints.

Key words

micropropagation bacterization mycorrhization stress resistance responses