Article

Folia Microbiologica

, Volume 35, Issue 3, pp 251-265

First online:

Continuous growth ofCandida utilis under periodic change of growth-limiting substrate

  • I. G. MinkevichAffiliated withInstitute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences
  • , M. SobotkaAffiliated withInstitute of Microbiology, Czechoslovak Academy of Sciences
  • , D. VranáAffiliated withInstitute of Microbiology, Czechoslovak Academy of Sciences
  • , I. HavlikAffiliated withInstitute of Microbiology, Czechoslovak Academy of Sciences

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Abstract

A periodic change of limitation by glucose and ammonia was effected during continuous cultivation ofCandida utilis. Values of feed parameters providing periodic nitrogen limitation were established. Cell biomass yield, macromolecular composition and parameters of individual cells (cell mass, budding percentage and cell-wall polysaccharide per surface square unit) were examined. The cyclic regime was found to result in culture synchrony. Parametêrs obtained on the basis of cell counts displayed a high sensitivity to changes in limitation where as the remaining parameters were less sensible.