Folia Microbiologica

, Volume 47, Issue 3, pp 283-286

First online:

Decolorization of synthetic textile dyes by lignin peroxidase ofPhanerochaete chrysosporium

  • P. VermaAffiliated withPost-Graduate Department of Biosciences, Sardar Patel University
  • , D. MadamwarAffiliated withPost-Graduate Department of Biosciences, Sardar Patel University

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Neem hull waste (containing a high amount of lignin and other phenolic compounds) was used for lignin peroxidase production byPhanerochaete chrysosporum under solid-state fermentation conditions. Maximum decolorization achieved by partially purified lignin peroxidase was 80% for Porocion Brilliant Blue HGR, 83 for Ranocid Fast Blue, 70 for Acid Red 119 and 61 for Navidol Fast Black MSRL. The effects of different concentrations of veratryl alcohol, hydrogen peroxide, enzyme and dye on the efficiency of decolorization have been investigated. Maximum decolorization efficiency was observed at 0.2 and 0.4 mmol/L hydrogen peroxide, 2.5 mmol/L veratryl alcohol and pH 5.0 after a 1-h reaction, using 50 ppm of dyes and 9.96 mkat/L of enzyme.