Mitochondria—Tool for taxonomic identification of yeasts fromSaccharomyces sensu stricto complex
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- Šoltésová, A., Špírek, M., Horváth, A. et al. Folia Microbiol (2000) 45: 99. doi:10.1007/BF02817406
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Mitochondrial genomes ofSaccharomyces and close relatives previously used for transplacement of mitochondria toS. cerevisiae were examined. The origins of replication in mitochondrial DNA, the presence of nuclear and mitochondrial polymorphic loci and the ability to produce mitochondrial respiration-deficient mutants were used to reclassify some collection yeasts and to assign others into four separate subgroups. The first included isolates identical toSaccharomyces cerevisiae (S. italicus, S. oviformis, S. chevalieri andS. capensis) which possess 5 or more replication origins. The second group consists ofS. paradoxus (var.douglasii) mitochondrial genome with the equal number ofori sequences but incompatible mitochondria. The third group representsSaccharomyces sensu stricto petite-positive species (S. carlsbergensis, S. heterogenicus. S. uvarum. S. willianus) with 1–2 origins of replication significantly different fromS. cerevisiae. In addition, the locus between tRNAfMet and tRNAPro is about one-half of the 1400 bp members of S. cerevisiae complex. The last group includes isolates that do not belong toSaccharomyces sensu stricto group as they are petite-negative and devoid of anyS. cerevisiae-like replication origins.