Article

Folia Microbiologica

, Volume 44, Issue 5, pp 491-502

First online:

Cloning of the putative aldehyde dehydrogenase,aldA, gene fromStreptomyces aureofaciens

  • O. SprušanskýAffiliated withInstitute of Molecular Biology, Slovak Academy of Sciences
  • , D. HomérováAffiliated withInstitute of Molecular Biology, Slovak Academy of Sciences
  • , B. ŠevčíkováAffiliated withInstitute of Molecular Biology, Slovak Academy of Sciences
  • , J. KormanecAffiliated withInstitute of Molecular Biology, Slovak Academy of Sciences Email author 

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Abstract

AStreptomyces aureofaciens gene,gap, encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was previously identified. Hybridization studies suggested the presence of a secondgap gene inS. aureofaciens. To clone the gene,S. aureofaciens subgenomic library was screened with an oligonucleotide probe encoding a peptide motif conserved in all GAPDH. 3352 bp positiveBamHI fragment was identified, the length of which correlated with the hybridization signal. The nucleotide sequence of the fragment was determined, and analysis of the sequence revealed the presence of three open reading frames (ORF). However, none of the genes coded for GAPDH. All three genes formed an operon, consisting of geneorf251, with a high homology to a conserved gene present only in archæabacteria, and thealdA andadhA genes homologous to various eukaryotic and prokaryotic aldehyde- and alcohol-dehydrogenases, with maximum homology to the phenylacetaldehyde dehydrogenases and arylalcohol dehydrogenases, respectively.