The in vitro formation of recombinant τ polymers
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- Ledesma, M.D., Medina, M. & Avila, J. Molecular and Chemical Neuropathology (1996) 27: 249. doi:10.1007/BF02815107
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τ Isolated from paired helical filaments, aberrant structures that appear in Alzheimer disease (AD) patients’ brains, show at least two posttranslational modifications: phosphorylation (Grundke-Iqbal et al., 1986; Ihara et al., 1986) and glycation (Ledesma et al., 1994; Yan et al., 1994). To test whether these modifications could affect the capacity of τ to self-aggregate, recombinant τ was phosphorylated and glycated, and its capacity to form polymers analyzed. Our results indicate that on phosphorylation and glycation, the capacity of τ to form aggregates increases, and that glycation of τ could stabilize the assembled polymers and could facilitate formation of bundles from these polymers.