Folia Microbiologica

, Volume 36, Issue 4, pp 343–346

Production of laccase byCurvularia sp.

  • U. C. Banerjee
  • R. M. Vohra
Papers

DOI: 10.1007/BF02814506

Cite this article as:
Banerjee, U.C. & Vohra, R.M. Folia Microbiol (1991) 36: 343. doi:10.1007/BF02814506

Abstract

ACurvularia sp. isolated from soil was found to contain laccase activity toward guaiacol as substrate. The organism produced an extracellular laccase in a medium containing yeast extract, peptone and dextrose. Initial medium pH 4.0 and cultivation temperature 30°C were found to be most suitable for maximum enzyme production. The optimum pH and temperature for laccase activity were found to be 5.2 and 50°C, respectively. Under optimum conditions, the enzyme had aKm (guaiacol) of 0.75 mmol/L and aV of 1.50 CU min−1 ml−1. Some divalent metal ions inhibited laccase activity at very low concentrations.

Copyright information

© Folia Microbiologica 1991

Authors and Affiliations

  • U. C. Banerjee
    • 1
  • R. M. Vohra
    • 1
  1. 1.Institute of Microbial TechnologyChandigarhIndia