Plant Molecular Biology Reporter

, Volume 20, Issue 2, pp 161–175

Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of juncaceae

Authors

    • Institute of BotanyAcademy of Sciences
  • Jan Kirschner
    • Institute of BotanyAcademy of Sciences
  • Ĉestmír Vlĉek
    • Centre for Integrated Genomics and Institute of Molecular GeneticsAcademy of Sciences
Protocols

DOI: 10.1007/BF02799431

Cite this article as:
Drábková, L., Kirschner, J. & Vlĉek, Ĉ. Plant Mol Biol Rep (2002) 20: 161. doi:10.1007/BF02799431

Abstract

Seven DNA extraction protocols were used to obtain DNA from herbarium specimens ofJuncus andLuzula (Juncaceae) of various ages. DNA of historical samples is difficult to extract, and the extracts are seldom of good quality. The quality of DNA obtained was estimated by using a spectrophotometer to measure the A260/280 absorbance ratio. The total DNA yield was measured by a fluorometer. The results indicate the success of using both mixer mill grinding and a DNeasy Plant Kit. Another extraction protocol (grinding with mortar and pestle, using liquid nitrogen) yielded DNA from many samples. Modified CTAB extraction, with a lengthy precipitation, usually provided good amounts of DNA. Other protocols did not give satisfactory results.

Key words

cpDNADNA extractionherbarium specimensJuncaceaeJuncusLuzulaPCR amplification

Abbreviations

CTAB

hexadecyltrimetylammonium bromide

EtOH

ethanol

Copyright information

© International Society for Plant Molecular Biology 2002