Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of juncaceae
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Seven DNA extraction protocols were used to obtain DNA from herbarium specimens ofJuncus andLuzula (Juncaceae) of various ages. DNA of historical samples is difficult to extract, and the extracts are seldom of good quality. The quality of DNA obtained was estimated by using a spectrophotometer to measure the A260/280 absorbance ratio. The total DNA yield was measured by a fluorometer. The results indicate the success of using both mixer mill grinding and a DNeasy Plant Kit. Another extraction protocol (grinding with mortar and pestle, using liquid nitrogen) yielded DNA from many samples. Modified CTAB extraction, with a lengthy precipitation, usually provided good amounts of DNA. Other protocols did not give satisfactory results.
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- Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of juncaceae
Plant Molecular Biology Reporter
Volume 20, Issue 2 , pp 161-175
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- DNA extraction
- herbarium specimens
- PCR amplification
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- Author Affiliations
- 1. Institute of Botany, Academy of Sciences, Zámek 1, 252 43, Průhonice, Czech Republic
- 2. Centre for Integrated Genomics and Institute of Molecular Genetics, Academy of Sciences, Flemingovo nám. 2, 166 37, Prague 6, Czech Republic