Applied Biochemistry and Biotechnology

, Volume 11, Issue 6, pp 433–443

Recycle of the cellulase— enzyme complex after hydrolysis of steam-exploded wood

  • L. S. Clesceri
  • A. P. Sinitsyn
  • A. M. Saunders
  • H. R. Bungay
Original Articles

DOI: 10.1007/BF02798639

Cite this article as:
Clesceri, L.S., Sinitsyn, A.P., Saunders, A.M. et al. Appl Biochem Biotechnol (1985) 11: 433. doi:10.1007/BF02798639

Abstract

Cellulases can be recovered in high yields by contacting fresh substrate with hydrolysis filtrate and by extraction of spent hydrolysis residue with pH 7 buffer. Recycled enzymes give hydrolysis rates about equal to those with fresh enzymes. Steam-exploded wood (SEW) is washed with water to remove sugars and byproducts from breakdown of hemicellulose, and recycle of enzymes proceeds better if lignin is also removed prior to hydrolysis. Oven drying of SEW interferes with recycle, and the recovery of enzymes is only one-half of that with SEW that is kept moist.

Effectiveness of enzyme recovery depends on the completeness of hydrolysis, as determined by contact time and enzyme concentration. For cost-effective operation, enzyme should not be recovered until appreciable filter paper activity and carboxylmethylcellulase activity appear in the hydrolysate.

Index Entries

Cellulase-enzyme complex, recycle ofsteam-exploded wood, hydrolysis ofcellulaseligninhemicelluloseglucoseenzyme productionfuel-grade alcohol

Copyright information

© Humana Press Inc 1985

Authors and Affiliations

  • L. S. Clesceri
    • 1
  • A. P. Sinitsyn
    • 1
    • 2
  • A. M. Saunders
    • 1
  • H. R. Bungay
    • 1
  1. 1.Rensselaer Polytechnic InstituteTroy
  2. 2.Department of ChemistryMoscow State UniversityUSSR