Original Articles

Applied Biochemistry and Biotechnology

, Volume 11, Issue 6, pp 433-443

First online:

Recycle of the cellulase— enzyme complex after hydrolysis of steam-exploded wood

  • L. S. ClesceriAffiliated withRensselaer Polytechnic Institute
  • , A. P. SinitsynAffiliated withRensselaer Polytechnic InstituteDepartment of Chemistry, Moscow State University
  • , A. M. SaundersAffiliated withRensselaer Polytechnic Institute
  • , H. R. BungayAffiliated withRensselaer Polytechnic Institute

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Cellulases can be recovered in high yields by contacting fresh substrate with hydrolysis filtrate and by extraction of spent hydrolysis residue with pH 7 buffer. Recycled enzymes give hydrolysis rates about equal to those with fresh enzymes. Steam-exploded wood (SEW) is washed with water to remove sugars and byproducts from breakdown of hemicellulose, and recycle of enzymes proceeds better if lignin is also removed prior to hydrolysis. Oven drying of SEW interferes with recycle, and the recovery of enzymes is only one-half of that with SEW that is kept moist.

Effectiveness of enzyme recovery depends on the completeness of hydrolysis, as determined by contact time and enzyme concentration. For cost-effective operation, enzyme should not be recovered until appreciable filter paper activity and carboxylmethylcellulase activity appear in the hydrolysate.

Index Entries

Cellulase-enzyme complex, recycle of steam-exploded wood, hydrolysis of cellulase lignin hemicellulose glucose enzyme production fuel-grade alcohol