In Vitro

, Volume 12, Issue 3, pp 180–191

Human colonic adenocarcinoma cells

I. Establishment and description of a new line
  • Baldwin H. Tom
  • Lynne P. Rutzky
  • Milda M. Jakstys
  • Ryoichi Oyasu
  • Celia I. Kaye
  • Barry D. Kahan
Article

DOI: 10.1007/BF02796440

Cite this article as:
Tom, B.H., Rutzky, L.P., Jakstys, M.M. et al. In Vitro (1976) 12: 180. doi:10.1007/BF02796440

Summary

A series of human colonic epithelial cell lines have been cultured from a single patient: LS-180 the original adenocarcinoma, LS-174T a trypsinized variant, and normal colonic tissue. The malignant cells, 20 to 40 µm in diameter and oval to polygonal, exhibited characteristics of normal colonic mucosal cells, namely, abundant microvilli prominent in secretory cells, and the presence of intracytoplasmic mucin vacuoles. The cultured adenocarcinoma cells, but not normal, demonstrated neoplastic properties by producing high levels of carcinoembryonic antigen (CEA) and by the ability to be propagated in hamster cheek pouches and in immunodeprived mice. The CEA production by the newly established line LS-180 released 900 times more CEA per cell into the culture medium and bore 30 times more cell-associated material than the established line, HT-29. These cell lines may permit detection of distinctive chemical, physiological, pharmacologic, and immunologic characteristics of neoplastic colonic cells.

Key words

human cancer colon cell line morphology carcinoembryonic antigen light and electron microscopy 

Copyright information

© Tissue Culture Association 1976

Authors and Affiliations

  • Baldwin H. Tom
    • 1
    • 2
  • Lynne P. Rutzky
    • 1
    • 2
  • Milda M. Jakstys
    • 1
    • 2
  • Ryoichi Oyasu
    • 1
    • 2
  • Celia I. Kaye
    • 1
    • 2
  • Barry D. Kahan
    • 1
    • 2
  1. 1.Laboratory of Surgical Immunology of the Departments of Surgery and Physiology, Department of PathologyNorthwestern University Medical Center, Veterans Administration Lakeside HospitalChicago
  2. 2.Division of Genetics and Metabolism, Department of PediatricsCook County HospitalChicago

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