In Vitro - Plant

, Volume 12, Issue 12, pp 821–832

Conditions affecting primary cell cultures of functional adult rat hepatocytes

II. Dexamethasone enhanced longevity and maintenance of morphology
  • Brian A. Laishes
  • Gary M. Williams

DOI: 10.1007/BF02796367

Cite this article as:
Laishes, B.A. & Williams, G.M. In Vitro Cell.Dev.Biol.-Plant (1976) 12: 821. doi:10.1007/BF02796367


Primary monolayer cell cultures of adult rat hepatocytes underwent change in morphology and substantial cell loss between 1 and 3 days postinoculation. Dexamethasone-supplementation (1µM) of the culture medium maintained the polygonal epithelial morphology of the hepatocytes and increased longevity such that over 80% of the cells survived for 3 days and at least 30% for 8 or 9 days. This enhancement of survival was obtained up to 48 hr postinoculation, but the earlier the time of dexamethasone supplementation the greater the effect. Removal of dexamethasone resulted in a decrease in longevity. The positive effect of dexamethasone on longevity was observed following dexamethasone replacement of insulin in supplemented cultures, but the combination of insulin and dexamethasone resulted in poorer survival than with dexamethasone alone. The results are interpreted to indicate that dexamethasone provided a requirement of the in vitro environment for survival and suggest that elaboration of a complex medium is required to maintain hepatocytes in culture.

Key words

hepatocyte culturesdexamethasoneinsulintissue cultureliverperfusion

Copyright information

© Society for In Vitro Biology 1977

Authors and Affiliations

  • Brian A. Laishes
    • 1
  • Gary M. Williams
    • 1
  1. 1.Fels Research Institute and Department of PathologyTemple University School of MedicinePhiladelphia
  2. 2.Department of PathologyUniversity of TorontoTorontoCanada
  3. 3.Naylor Dana Institute for Disease PreventionAmerican Health FoundationValhalla