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Time course effects of vanadium supplement on cytosolic reduced glutathione level and glutathione S-transferase activity

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Abstract

The influence of vanadium, an important dietary micronutrient, was evaluated on the cytosolic reduced glutathione (GSH) content and glutathione S-transferase (GST) activity in several rat target tissues. Supplementation of drinking water with vanadium at the level of 0.2 or 0.5 ppm for 4, 8, or 12 wk was found to increase the GSH level with a concomitant elevation in GST activity in the liver followed by small intestine mucosa, large intestine mucosa, and kidney. The results were almost dose-dependent and mostly pronounced with 0.5 ppm vanadium after 12 wk of its continuous supplementation. Neither the GSH level nor GST activity was significantly altered in forestomach and lung following vanadium supplementation throughout the study. The levels of vanadium that were found to increase the content of GSH and activity of GST in the liver, intestine, and kidney did not exert any toxic manifestation was evidenced from water and food consumption as well as the growth responses of the experimental animals. Moreover, these doses of vanadium did not impair either hepatic or renal functions as they did not alter the serum activities of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), sorbitol dehydrogenase (SDH), as well as serum urea and creatinine levels. All these results clearly indicate that vanadium under the doses employed in our study has a significant inducing role on GSH content with a concurrent elevation in GST activity in the liver and specific extrahepatic tissues without any apparent sign of cytotoxicity. This attribute of vanadium may have a greater importance in terms of biotransformation and detoxification of xenobiotics, including carcinogens. In addition, since the ability to afford an increment in the endogenous GSH-GST pool by anticarcinogenic natural substances has been found to correlate with their activity to inhibit neoplastic transformation, the trace element vanadium may be considered as a novel anticancer agent.

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References

  1. R. J. French and P. J. Jones,Life Sci. 52, 339 (1993).

    Article  PubMed  CAS  Google Scholar 

  2. B. R. Nechay, L. B. Ninninga, S. E. Nechay, R. L. Post, J. J. Grantham, I. G. Macara, L. F. Kubena, T. D. Phillips, and F. H. Nielsen,Fed. Proc. 42, 123 (1986).

    Google Scholar 

  3. D. J. D. Nicholas,Trace Elements in Soil-Plant-Animal Systems, D. J. D. Nicholas and A. R. Egans, eds., Academic, New York, pp. 181–182 (1975).

    Google Scholar 

  4. F. Bernheim and M. L. C. Bernheim,J. Biol. Chem. 127, 353 (1939).

    CAS  Google Scholar 

  5. R. Soremark and S. Ulberg,Use, of Radioisotopes in Animal Biology and the Medical Sciences, vol. 2, M. Fried, ed., Academic, New York, pp. 1–20. (1962).

    Google Scholar 

  6. I. G. Macara,Trends Biochem. Sci. 5, 92 (1980).

    Article  CAS  Google Scholar 

  7. E. Sabbioni, L. Clerici, and A. Brazzelli,J. Toxicol. Environ. Health 12, 737 (1983)

    Article  PubMed  CAS  Google Scholar 

  8. M. A. Serra, E. Sabbioni, A. Marchesini, A. Pintar, and M. Valoti,Biol. Trace Elem. Res. 23, 151 (1990).

    CAS  Google Scholar 

  9. E. G. De Master, and R. A. MitchellBiochemistry 12, 3616 (1973).

    Article  Google Scholar 

  10. D. C. Crans, J. Gonzales, C. H. Wong, and D. G. Druckhammer, presented at the 19th National Meetings of the American Chemical Society, New Orleans, LA, September, INOR 344 (1987).

  11. A. F. Nour-Eldeen, M. M. Craig, and M. J. Gresser,J. Biol. Chem. 260, 6836 (1985).

    PubMed  CAS  Google Scholar 

  12. X. Shi and N. S. Dalal,Arch. Biochem. Biophys. 278, 288 (1990).

    Article  PubMed  CAS  Google Scholar 

  13. A. Bishayee, S. Bhattacharjee, A. Chakraborty, K. K. Banerjee, and M. Chatterjee,Indian Med. Gez. 75, 299 (1991).

    Google Scholar 

  14. A. Bishayee, and M. Chatterjee,J. Inorg. Biochem. 54, 277 (1994).

    Article  CAS  Google Scholar 

  15. A. Chakraborty, S. Bhattacharjee, and M. Chatterjee,Bull. Environ. Contam. Toxicol. 54, 281 (1995).

    Article  PubMed  CAS  Google Scholar 

  16. A. Chakraborty, S. Bhattacharjee, and M. Chatterjee,Phill J. Sci., in press.

  17. E. Sabbioni, G. Pozzi, A. Pintar, L. Casella, and S. Garattini,Carcinogenesis 12, 47 (1991).

    Article  PubMed  CAS  Google Scholar 

  18. E. M. Kosower,Glutathione: Metabolism and Function, I. M. Arias and W. B. Jakoby, eds., Raven, New York, pp. 1–15 (1976).

    Google Scholar 

  19. S. C. Sharma and C. P. Siegers,Neunyn-Schmiedeberg's Arch. Pharmacol. 325 (suppl.), R25 (1984).

    Google Scholar 

  20. J. Donaldson, R. Hemming, and F. Labella,Can. J. Physiol. Pharmacol. 63, 196 (1985).

    PubMed  CAS  Google Scholar 

  21. M. A. at Bayati, S. N. Gire, O. G. Raabe, L. S. Rosenblatt, and M. Shifrine,J. Environ. Pathol. Toxicol. Oncol. 9, 435 (1989).

    Google Scholar 

  22. R. P. Sharma, R. A. Coulombe, Jr., and B. Srisuchart,Biochem. Pharmacol. 35, 461 (1986).

    Article  PubMed  CAS  Google Scholar 

  23. G. L. Ellman,Arch. Biochem. Biophys. 82, 70 (1959).

    Article  PubMed  CAS  Google Scholar 

  24. W. H. Habig, M. J. Pabst, and W. B. Jakoby,J. Biol. Chem. 249, 7130 (1974).

    PubMed  CAS  Google Scholar 

  25. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall,J. Biol. Chem. 193, 265 (1951).

    PubMed  CAS  Google Scholar 

  26. H. U. Bergmeyer and E. Brent,Methods of Enzymatic Analysis, vol. 2, H. U. Bergmeyer, ed., Verlag Chemie Weinhein, Academic, New York, pp. 735–762 (1974).

    Google Scholar 

  27. U. Gerlach and W. Hiby,Methods of Enzymatic Analysis, vol. 2, H. U. Bergmeyer, ed., Verlag Chemie Weinhein, Academic, New York, pp. 569–573 (1974).

    Google Scholar 

  28. S. Natelson,Microtechniques in Clinical Chemistry for Routine Laboratory, Springfield, IL (1957).

  29. J. A. Owen, B. Iggo, F. J. Scandrett, and C. P. Stewart,Biochem. J. 58, 426 (1954).

    PubMed  CAS  Google Scholar 

  30. L. F. Chasseaud,Adv. Cancer Res. 29, 175 (1979).

    Article  PubMed  CAS  Google Scholar 

  31. S. Uhlig and A. Wendel,Life Sci. 51, 1083 (1992).

    Article  PubMed  CAS  Google Scholar 

  32. A. M. Benson, Y.-N. Cha, E. Bueding, H. S. Heine, and P. Talalay,Cancer Res. 39, 2971 (1979).

    PubMed  CAS  Google Scholar 

  33. V. L. Sparnins, P. L. Venegas, and L. W. Wattenberg,J. Natl. Cancer Inst. 68, 493 (1982).

    PubMed  CAS  Google Scholar 

  34. L. K. T. Lam, V. L. Sparnins, and L. W. Wattenberg,Cancer Res. 42, 1193 (1982).

    PubMed  CAS  Google Scholar 

  35. H. L. Newmark,Can. J. Physiol. Pharmacol. 65, 461 (1987).

    PubMed  CAS  Google Scholar 

  36. V. L. Sparnins and L. W. Wattenberg,J. Natl. Cancer Inst. 66, 769 (1981).

    PubMed  CAS  Google Scholar 

  37. L. W. Wattenberg,Prog. Exp. Tumor Res. 14, 89 (1971).

    PubMed  CAS  Google Scholar 

  38. E. C. Miller and J. A. Miller,Chemical Carcinogens, C. E. Searle, ed., American Chemical Society, Washington, DC, pp. 732–750 (1976).

    Google Scholar 

  39. D. M. Canning and A. B. C. Lansdowne,Toxic Hazards in Food, Croom Helm, London (1983).

    Google Scholar 

  40. M. D. Maines and A. Kappas,Proc. Natl. Acad. Sci. USA 74, 1875 (1977).

    Article  PubMed  CAS  Google Scholar 

  41. S. H. Oh, J. T. Deagen, P. D. Whanger, and P. H. Weswig,Am. J. Physiol. 234, E282-E284 (1978).

    PubMed  CAS  Google Scholar 

  42. M. Webb and K. Cain,Biochem. Pharmacol. 31, 137 (1982).

    Article  PubMed  CAS  Google Scholar 

  43. G.-q. Zheng, P. M. Kenney, and L. K. T. Lam,J. Agric. Food Chem. 40, 107 (1992).

    Article  CAS  Google Scholar 

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  1. Division of Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, 700 032, Calcutta, India

    Anupam Bishayee & Malay Chatterjee

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  1. Anupam Bishayee
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  2. Malay Chatterjee
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Bishayee, A., Chatterjee, M. Time course effects of vanadium supplement on cytosolic reduced glutathione level and glutathione S-transferase activity. Biol Trace Elem Res 48, 275–285 (1995). https://doi.org/10.1007/BF02789409

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  • DOI: https://doi.org/10.1007/BF02789409

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