PCR-mediated recombination and mutagenesis
- Robert M. Horton
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Gene Splicing by Overlap Extension (gene SOEing) is a sequence-independent method for site-directed mutagenesis and/or recombination of DNA molecules. It is based on the idea that a PCR product can be engineered by adding or changing sequences at its ends so that the product can itself be used to prime DNA synthesis in a subsequent overlap-extension reaction to create mutant or recombinant molecules. As the engineered genes are created in vitro without reliance on host organisms or restriction sites, gene SOEing provides a powerful and versatile tool for genetic investigation and engineering.
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- PCR-mediated recombination and mutagenesis
Volume 3, Issue 2 , pp 93-99
- Cover Date
- Print ISSN
- Online ISSN
- Humana Press
- Additional Links
- Polymerase chain reaction
- overlap extension
- recombinant PCR
- gene SOEing
- sitedirected mutagenesis
- Industry Sectors
- Robert M. Horton (1)
- Author Affiliations
- 1. Department of Biochemistry, College of Biological Sciences, 1479 Gortner Ave., 55108, St. Paul, MN