Biological Trace Element Research

, Volume 61, Issue 3, pp 263–275

Separation of cellular iron containing compounds by electrophoresis

  • Daniel Vyoral
  • Jirí Petrák
  • Antonín Hradilek
Original Articles

DOI: 10.1007/BF02789087

Cite this article as:
Vyoral, D., Petrák, J. & Hradilek, A. Biol Trace Elem Res (1998) 61: 263. doi:10.1007/BF02789087

Abstract

High resolution separation of metalloproteins and other iron compounds based on native gel electrophoresis followed by59Fe autoradiography is described. Lysates of mouse spleen erythroid cells metabolically labeled with59Fe-transferrin were separated on 3–20% polyacrylamide gradient gels in the presence of Triton X100 and detected by autoradiography. In addition to ferritin and hemoglobin, several compounds characterized by their binding of iron under different conditions were described. Iron chelatable by desferrioxamine migrated in the region where several high-molecular weight compounds were detected by silver staining. The technique is nondissociative, allowing identification of iron compounds with the use of specific antibodies. Cellular iron transport and the action of iron chelators on specific cellular targets can be investigated in many small biological samples in parallel.

Index Entries

59Feironchelatable ironferritinhemedesferrioxaminenative electrophoresis

Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  • Daniel Vyoral
    • 1
  • Jirí Petrák
    • 1
  • Antonín Hradilek
    • 1
  1. 1.Institute of Hematology and Blood TransfusionPrague 2Czech Republic