Applied Biochemistry and Biotechnology

, Volume 75, Issue 2, pp 163–173

Effects of glycerol concentration and pH on growth of recombinantPichia pastoris yeast

  • V. Chiruvolu
  • K. Eskridge
  • J. Cregg
  • M. Meagher
Original Ariticle

DOI: 10.1007/BF02787771

Cite this article as:
Chiruvolu, V., Eskridge, K., Cregg, J. et al. Appl Biochem Biotechnol (1998) 75: 163. doi:10.1007/BF02787771


Batch fermentations were used to study the effect of different glycerol concentrations and pH conditions on growth of recombinantPichia pastoris. Two strains ofP. pastoris were used: a wild-type in methanol utilization (Mut+) and a mutant defective in methanol utilization (Mut-). Under constant pH conditions of 5.0, glycerol concentrations up to 12% were efficiently utilized. Cell yield (Yx/s) of about 0.8 and a final cell density of about 95 g/L (dry cell) were achieved. However, there were significant differences (probability [Pr]> F 0.0351) in specific growth rates between the initial glycerol concentrations of 2, 7, and 12%. When fermentations were conducted without pH control, growth continued until the pH had decreased to about 2.5. Growth stopped at pH 2.2 with uncontrolled pH, and residual glycerol concentrations were greater than 2%. As a result, Yx/s decreased to about 0.3. There were no differences between Mut+ and Mut- strains during cell growth on glycerol.

Index entries

Pichia pastorisalcohol oxidaseMut+Mut-specific growth rate

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • V. Chiruvolu
    • 1
  • K. Eskridge
    • 2
  • J. Cregg
    • 3
  • M. Meagher
    • 4
  1. 1.Scios,Inc.Mountain View
  2. 2.Department of BiometryUniversity of Nebraska-Lincoln
  3. 3.Department of Chemistry,Biochemistry and Molecular BiologyOregon Graduate Institute of Science and TechnologyPortland
  4. 4.Biological Process Development Facility, Departments of Food Science and Technology and Biological Systems EngineeringUniversity of NebraskaLincoln