, Volume 55, Issue 3, pp 261-273

Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart. (Camaratu Bean)

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Abstract

A quantitatively main molecular form ofCratylia mollis lectin, isoform 1 (iso 1) was purified by affinity chromatography on Sephadex G-75, followed by ion exchange chromatography on CM-cellulose. Another lectin form was identified in the latter step. Iso 1 is specific for glucose/mannose, with a main subunit of 31 kDa mol wt; the native protein is basic (pI 8.5-8.6) and the constituent polypeptides had a pI range of 5.15–7.75. An antibody to the protein was raised in a rabbit, and the conjugate was active in an immunosorbent assay.