Applied Biochemistry and Biotechnology

, Volume 55, Issue 3, pp 261–273

Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart. (Camaratu Bean)

  • Maria T. S. Correia
  • Luana C. B. B. Coelho
Original Articles

DOI: 10.1007/BF02786865

Cite this article as:
Correia, M.T.S. & Coelho, L.C.B.B. Appl Biochem Biotechnol (1995) 55: 261. doi:10.1007/BF02786865


A quantitatively main molecular form ofCratylia mollis lectin, isoform 1 (iso 1) was purified by affinity chromatography on Sephadex G-75, followed by ion exchange chromatography on CM-cellulose. Another lectin form was identified in the latter step. Iso 1 is specific for glucose/mannose, with a main subunit of 31 kDa mol wt; the native protein is basic (pI 8.5-8.6) and the constituent polypeptides had a pI range of 5.15–7.75. An antibody to the protein was raised in a rabbit, and the conjugate was active in an immunosorbent assay.

Index Entries

Lectinisoformlectin purificationantilectin IgGantilectin IgG-peroxidase

Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Maria T. S. Correia
    • 1
  • Luana C. B. B. Coelho
    • 1
  1. 1.Departamento de Bioquímica, Centro de Ciências BiológicasUniversidade Federal de Pernambuco, UFPEPEBrazil