Immunologic Research

, Volume 16, Issue 1, pp 115–119

Multiplicity of uses of monoclonal antibodies that define papillomavirus linear immunodominant epitopes

  • A. Bennett Jenson
  • Michelle C. Jenson
  • Lex Cowsert
  • Shin-je Ghim
  • John P. Sundberg
Article

DOI: 10.1007/BF02786327

Cite this article as:
Jenson, A.B., Jenson, M.C., Cowsert, L. et al. Immunol Res (1997) 16: 115. doi:10.1007/BF02786327

Abstract

During the last 10 yr, we have derived monoclonal antibodies from animals immunized with denatured bovine papillomaviruses type 1 major capsid (L1) protein, mapped their corresponding immunodominant epitopes to within a single amino acid (aa), and compared the reactivity of authentic L1 proteins to the predicted response by collinear analysis of the aa sequences of the same and other papillomaviruses (PVs). The data obtained from this approach has provided us with new insights into the sensitivity and specificity of the antibody response to viral proteins. We have included here some observations and conclusions that appear to be generic for the immune response, some of which might have applications for working with linear epitopes in other experimental systems.

Key Words

Immunodominant epitopesPapillomavirusTrimeric and pentameric peptides

Copyright information

© Humana Press Inc 1997

Authors and Affiliations

  • A. Bennett Jenson
    • 1
  • Michelle C. Jenson
    • 1
  • Lex Cowsert
    • 2
  • Shin-je Ghim
    • 2
  • John P. Sundberg
    • 3
  1. 1.Department of PathologyGeorgetown University School of MedicineWashington, DC
  2. 2.ISISPharmaceutical IncCarlsbad
  3. 3.The Jackson LaboratoryBar Harbor