Applied Biochemistry and Biotechnology

, Volume 68, Issue 1, pp 21–40

Pretreatment of yellow poplar sawdust by pressure cooking in water

  • Joe Weil
  • Ayda Sarikaya
  • Shiang-Lan Rau
  • Joan Goetz
  • Christine M. Ladisch
  • Mark Brewer
  • Rick Hendrickson
  • Michael R. Ladisch
Original Articles

DOI: 10.1007/BF02785978

Cite this article as:
Weil, J., Sarikaya, A., Rau, S. et al. Appl Biochem Biotechnol (1997) 68: 21. doi:10.1007/BF02785978

Abstract

The pretreatment of yellow poplar wood sawdust using liquid water at temperatures above 220°C enhances enzyme hydrolysis. This paper reviews our prior research and describes the laboratory reactor system currently in use for cooking wood sawdust at temperatures ranging from 220 to 260°C. The wood sawdust at a 6–6.6% solid/liquid slurry was treated in a 2 L, 304 SS, Parr reactor with three turbine propeller agitators and a proportional integral derivative (PID) controller, which controlled temperature within ±1°C. Heat-up times to the final temperatures of 220, 240, or 260°C were achieved in 60–70 min. Hold time at the final temperature was less than 1 min. A serpentine cooling coil, through which tap water was circulated at the completion of the run, cooled the reactor’s contents within 3 min after the maximum temperature was attained. A bottoms port, as well as ports in the reactor’s head plate, facilitated sampling of the slurry and measuring the pH, which changes from an initial value of 5 before cooking to a value of approx 3 after cooking. Enzyme hydrolysis gave 80–90% conversion of cellulose in the pretreated wood to glucose. Simultaneous saccharification and fermentation of washed, pretreated lignocellulose gave an ethanol yield that was 55% of theoretical. Untreated wood sawdust gave less than 5% hydrolysis under the same conditions.

Index Entries

Woodwater pretreatmentenzymehydrolysisfermentationpretreatmentcelluloseglucoseethanol

Copyright information

© Humana Press Inc 1997

Authors and Affiliations

  • Joe Weil
    • 1
    • 2
  • Ayda Sarikaya
    • 1
  • Shiang-Lan Rau
    • 1
    • 3
  • Joan Goetz
    • 3
  • Christine M. Ladisch
    • 3
  • Mark Brewer
    • 1
  • Rick Hendrickson
    • 1
  • Michael R. Ladisch
    • 1
    • 2
  1. 1.Laboratory of Renewable Resources EngineeringPurdue UniversityWest Lafayette
  2. 2.Department of Agricultural and Biological EngineeringPurdue UniversityWest Lafayette
  3. 3.Textile Science-CSRPurdue UniversityWest Lafayette