Article

Applied Biochemistry and Biotechnology

, Volume 61, Issue 1, pp 189-198

Cyclodextrins as hosts for poorly water-soluble compounds in enzyme catalysis

  • Roque BruAffiliated withDepartamento de Bioquimica y Biologia Molecular A, Facultad de Biologia, Universidad de Murcia
  • , Jose M. López-NicolásAffiliated withDepartamento de Bioquimica y Biologia Molecular A, Facultad de Biologia, Universidad de Murcia
  • , Estrella Núñez-DelicadoAffiliated withDepartamento de Bioquimica y Biologia Molecular A, Facultad de Biologia, Universidad de Murcia
  • , Dolores Nortes-RuipérezAffiliated withDepartamento de Bioquimica y Biologia Molecular A, Facultad de Biologia, Universidad de Murcia
  • , Alvaro Sánchez-FerrerAffiliated withDepartamento de Bioquimica y Biologia Molecular A, Facultad de Biologia, Universidad de Murcia
  • , Francisco Garciá-CarmonaAffiliated withDepartamento de Bioquimica y Biologia Molecular A, Facultad de Biologia, Universidad de Murcia

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Abstract

The capability of cyclodextrins to enhance greatly the solubility in water of poorly water-soluble substances makes them an ideal alter native for investigating the expression of enzyme activity with such substrates in aqueous solution. This capability is demonstrated by using soybean lipoxygenase with linoleic acid/β-cyclodextrin and di ethylstilbestrol/γ-cyclodextrin, and cholesterol oxidase with choles terol/methyl-β-cyclodextrin.

Index entries

Cyclodextrins linoleic acid diethylstilbestrol cholesterol lipoxygenase cholesterol oxidase