Applied Biochemistry and Biotechnology

, Volume 61, Issue 1, pp 189–198

Cyclodextrins as hosts for poorly water-soluble compounds in enzyme catalysis

Authors

  • Roque Bru
    • Departamento de Bioquimica y Biologia Molecular A, Facultad de BiologiaUniversidad de Murcia
  • Jose M. López-Nicolás
    • Departamento de Bioquimica y Biologia Molecular A, Facultad de BiologiaUniversidad de Murcia
  • Estrella Núñez-Delicado
    • Departamento de Bioquimica y Biologia Molecular A, Facultad de BiologiaUniversidad de Murcia
  • Dolores Nortes-Ruipérez
    • Departamento de Bioquimica y Biologia Molecular A, Facultad de BiologiaUniversidad de Murcia
  • Alvaro Sánchez-Ferrer
    • Departamento de Bioquimica y Biologia Molecular A, Facultad de BiologiaUniversidad de Murcia
  • Francisco Garciá-Carmona
    • Departamento de Bioquimica y Biologia Molecular A, Facultad de BiologiaUniversidad de Murcia
Article

DOI: 10.1007/BF02785701

Cite this article as:
Bru, R., López-Nicolás, J.M., Núñez-Delicado, E. et al. Appl Biochem Biotechnol (1996) 61: 189. doi:10.1007/BF02785701

Abstract

The capability of cyclodextrins to enhance greatly the solubility in water of poorly water-soluble substances makes them an ideal alter native for investigating the expression of enzyme activity with such substrates in aqueous solution. This capability is demonstrated by using soybean lipoxygenase with linoleic acid/β-cyclodextrin and di ethylstilbestrol/γ-cyclodextrin, and cholesterol oxidase with choles terol/methyl-β-cyclodextrin.

Index entries

Cyclodextrinslinoleic aciddiethylstilbestrolcholesterollipoxygenasecholesterol oxidase

Abbreviations

α-CD

alpha-cyclodextrin

β-CD

beta-cyclodextrin

mβ-CD

methyl-beta-cyclodextrin

hpβ-CD

2-hydroxypropylpropyl-beta-cyclodextrin

malβ-CD

maltosyl-beta-cyclodextrin

γCD

gamma-cyclodextrin

LA

linoleic acid

CMC

critical micelle concentration

DES

diethylstilbestrol

Copyright information

© Humana Press Inc 1997