Applied Biochemistry and Biotechnology

, Volume 44, Issue 3, pp 213–229

Contribution to the study of the alteration of lipase activity ofCandida rugosa by ions and buffers

  • M. J. Hernáiz
  • M. Rua
  • B. Celda
  • P. Medina
  • J. V. Sinisterra
  • J. M. Sánchez-Montero
Article

DOI: 10.1007/BF02779658

Cite this article as:
Hernáiz, M.J., Rua, M., Celda, B. et al. Appl Biochem Biotechnol (1994) 44: 213. doi:10.1007/BF02779658

Abstract

A semipurifiedC. rugosa lipase (LS) has been prepared from commercial lipase (LC) using an economical procedure. The presence of sugars and glycopeptides has been detected in LS and LC. Pure lipase only has covalently bonded sugars. The hydrolysis of olive oil catalyzed by LS and commercial lipase (LC) is sensitive to the presence of cations Na(I), Mg(II), Ca(II), and Ba(II) and to the nature of buffer. Highest enzyme activity is obtained with 0.1M Tris/HCl buffers and the combination of NaCl 0.11M and CaCl2 0.11M. Fluorescence spetroscopy analysis of LC, LS, and both pure isoenzymes lipases A and B, was used to analyze the interaction of the lipase with these effecttors. Inorganic cations Na or Ca do not interact with pure enzyme LA but do interact with LC and LS and do so slightly with LB. The organic cations (morfolinium ortris) interact with pure lipases. We postulate that the increase in the lipase activity produced by Na(I) or Ca(II) is related with interfacial phenomena, but the increase might be more specific in the hydrolysis of olive oil in the presence of Tris-HCl or morfoline-HCl buffer, owing to enzyme-buffer interaction.

Copyright information

© Humana Press Inc 1994

Authors and Affiliations

  • M. J. Hernáiz
    • 1
  • M. Rua
    • 2
  • B. Celda
    • 3
  • P. Medina
    • 3
  • J. V. Sinisterra
    • 3
  • J. M. Sánchez-Montero
    • 1
  1. 1.Department of Organic and Pharmaceutical Chemistry, Faculty of PharmacyUniversidad ComplutenseMadridSpain
  2. 2.Instituto de CatálisisCSICMadridSpain
  3. 3.Department of Physical Chemistry, Faculty of ChemistryUniversidad de ValenciaSpain