Mutants ofEscherichia coli defective in acid fermentation
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Wild typeE. coli ferments glucose to a mixture of ethanol and acetic, lactic, formic, and succinic acids. Mutants defective in acid production have now been isolated, including those defective in lactate dehydrogenase (LDH) or with excess alcohol dehydrogenase. These mutations had no phenotype without apfl mutation. Novel mutants affecting acetate metabolism were isolated by insertion of the fusion vector Mudl. TheseaceG mutants cannot grow anaerobically on glucose or aerobically on acetate yet lack the pleiotropic growth defects of previously knownpta/ack mutants. In some genetic backgrounds acetate negative mutations suppress the growth defects ofadh mutations. These results are discussed in terms of redox balance.
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- Mutants ofEscherichia coli defective in acid fermentation
Applied Biochemistry and Biotechnology
Volume 17, Issue 1-3 , pp 163-173
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- Humana Press
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- Anaerobic growth
- alcohol dehydrogenase
- factate fermentation
- acetic acid, redox balance
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