Plant Molecular Biology Reporter

, Volume 21, Issue 2, pp 177–178

An efficient protocol for genomic DNA extraction fromCitrus species

Authors

  • Yun-Jiang Cheng
    • National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular BreedingHuazhong Agricultural University
  • Wen-Wu Guo
    • National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular BreedingHuazhong Agricultural University
  • Hua-Lin Yi
    • National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular BreedingHuazhong Agricultural University
  • Xiao-Min Pang
    • National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular BreedingHuazhong Agricultural University
    • National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular BreedingHuazhong Agricultural University
Publish by Abstract

DOI: 10.1007/BF02774246

Cite this article as:
Cheng, Y., Guo, W., Yi, H. et al. Plant Mol Biol Rep (2003) 21: 177. doi:10.1007/BF02774246

Abstract

We describe a simple and efficient method for genomic DNA extraction from woody fruit crops containing high polysaccharide levels. This method involves a modified CTAB or SDS procedure employing a purification step to remove polysaccharides by using water-saturated ether and 1.25 M NaCl. Precipitation with an equal volume of isopropanol caused a DNA pellet to form. After being washed with 70% ethyl alcohol, the pellet easily dissolved in TE buffer. Using this method, DNA was extracted from samples of more than 1000Citrus spp., including young leaves, old leaves, frosted old leaves, withered old leaves, and callus lines. The average yield of DNA ranged from 50–500 μg/g of sample. DNA was suitable for PCR and RFLP analyses and long-term storage. Recently, the procedure was used to isolate DNA from withered old leaves of more than 20 tropical and subtropical fruit crops.

Key words

DNA extractionfruit cropspolysaccharidesRFLPwater-saturated ether
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© International Society for Plant Molecular Biology 2003