Plant Molecular Biology Reporter

, Volume 21, Issue 4, pp 339–348

Using SD-AFLP and MSAP to assess CCGG methylation in the banana genome

  • Franc-Christophe Baurens
  • François Bonnot
  • David Bienvenu
  • Sandrine Causse
  • Thierry Legavre
Commentary

DOI: 10.1007/BF02772583

Cite this article as:
Baurens, FC., Bonnot, F., Bienvenu, D. et al. Plant Mol Biol Rep (2003) 21: 339. doi:10.1007/BF02772583

Abstract

Two amplified fragment length polymorphism (AFLP)-derived techniques were used to assess methylation at CCGG sites in the banana genome. Assessment of these techniques revealed that, while amplification steps are very reproducible, the ligation step is more subject to variability. Overall, these techniques produced an error rate of 0.2% per analysed band. Statistical approach highlights the fact that sample duplication is necessary to produce reliable results. This study involved 18 primer pairs and found that in the banana genome, roughly 80% of CCGG sites are unmethylated, 5% are methylated at the internal cytosine, and 15% are methylated at the external or both cytosines.

Key words

amplified fragment length polymorphism banana, DNA methylation methylation-sensitive amplification polymorphism secondary digest-AFLP 

Abbreviations

AFLP

amplified fragment length polymorphism

CIAA

chloroform-isoamylalcohol

MATAB

mixed alkyl trimethyl ammonium bromide

MSAP

methylation-sensitive amplification polymorphism

RFLP

restriction fragment length polymorphism

SD-AFLP

secondary digest-AFLP

Copyright information

© International Society for Plant Molecular Biology 2003

Authors and Affiliations

  • Franc-Christophe Baurens
    • 1
  • François Bonnot
    • 2
  • David Bienvenu
    • 1
  • Sandrine Causse
    • 3
  • Thierry Legavre
    • 4
  1. 1.CIRAD AMIS BiotropUMR 1098 (BEPC)Montpellier Cedex 5France
  2. 2.CIRAD CPMontpellier Cedex 5France
  3. 3.CIRAD FLHORUMR 1098 (BEPC)Montpellier Cedex 5France
  4. 4.CIRAD AMIS BiotropUMR 1098 (BEPC)Montpellier Cedex 5France

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