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Plant Molecular Biology Reporter

, Volume 20, Issue 4, pp 425-425

First online:

High-throughput DNA extraction from forest trees

  • Mervyn ShepherdAffiliated withCooperative Research Centre for Sustainable Production Forestry, Centre for Plant Conservation Genetics, Southern Cross University Email author 
  • , Michael CrossAffiliated withCooperative Research Centre for Sustainable Production Forestry, Centre for Plant Conservation Genetics, Southern Cross University
  • , Rhonda L. StokoeAffiliated withCooperative Research Centre for Sustainable Production Forestry, Centre for Plant Conservation Genetics, Southern Cross University
  • , Leon J. ScottAffiliated withCooperative Research Centre for Sustainable Production Forestry, Centre for Plant Conservation Genetics, Southern Cross University
  • , Megan E. JonesAffiliated withCentre for Plant Conservation Genetics, Southern Cross University

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Abstract

It is difficult to extract pure high-quality DNA from trees, which may not be amenable to advances in extraction methods suitable for other plants. A new commercial high-throughput DNA extraction system, using a silica binding matrix for purification and a multisample mixer mill for tissue disruption, was evaluated for its suitability withEucalyptus spp.,Pinus spp., andAraucaria cunninghamii (hoop pine). DNA suitable for a range of molecular biology applications was successfully extracted from all genera. The method was highly reliable when tested in more than 500 preparations and could be adapted to different tree species with relatively minor modifications.

Key words

AFLP Araucaria Eucalyptus microsatellite Pinus