Journal of Assisted Reproduction and Genetics

, Volume 14, Issue 2, pp 111–119

Comparative flow cytometric analysis of the human sperm acrosome reaction using CD46 antibody and lectins

  • J. A. Carver-Ward
  • I. M. Moran-Verbeek
  • J. M. G. Hollanders
Genetics

DOI: 10.1007/BF02765780

Cite this article as:
Carver-Ward, J.A., Moran-Verbeek, I.M. & Hollanders, J.M.G. J Assist Reprod Genet (1997) 14: 111. doi:10.1007/BF02765780

Abstract

Purpose: Our purpose was to determine the most suitable marker for the human sperm acrosome reaction, based on detection of CD46 antibody binding compared with lectin binding.

Methods: Flow cytometric analysis of CD46 antibody versus lectins (PNA, PSA, and Con A) was used to quantify the acrosome reaction of human sperm.

Results: Neither PSA nor Con A was able to detect significant changes in the spontaneous and ionophore-induced acrosome reactions compared to CD46 antibody. However, PNA was found to exhibit a binding pattern similar to that observed with CD46 and could be used to quantify measurable changes in acrosomal response to ionophore, albeit of a lower magnitude than the responses detected by CD46.

Conclusions: We conclude that PNA binds to the inner acrosomal membrane of acrosome-reacted sperm and is suitable for use as a marker of the acrosome reaction by flow cytometry. Data are presented which clarify the assessment of the acrosome reaction when CD46 and lectins are used.

Key words

lectins CD46 flow cytometry acrosome 

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • J. A. Carver-Ward
    • 1
  • I. M. Moran-Verbeek
    • 2
  • J. M. G. Hollanders
    • 3
  1. 1.IVF Laboratory, Department of Pathology and Laboratory MedicineKing Faisal Specialist Hospital and Research CentreRiyadhSaudi Arabia
  2. 2.Department of Biological and Medical ResearchKing Faisal Specialist Hospital and Research CentreRiyadhSaudi Arabia
  3. 3.Department of Obstetrics and GynecologyKing Faisal Specialist Hospital and Research CentreRiyadhSaudi Arabia
  4. 4.Al Ain, Abu DhabiUAE

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