Molecular Biotechnology

, Volume 5, Issue 1, pp 39-47

First online:

Cloning single-stranded DNA

  • Joachim MessingAffiliated withWaksman Institute, Rutgers, The State University of NJ

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


DNA sequence and expression analyses have greatly benefited from using M13 and pUC derived cloning vectors and their polycloning sites. A chronology of the original concepts and experiments is reviewed.

Index Entries

M13mp and pUC history polycloning sites universal sequencing primer