Journal of Molecular Neuroscience

, Volume 8, Issue 2, pp 159–161

Cloning and identification of human syntaxin 5 as a synaptobrevin/VAMP binding protein

  • Veerasamy Ravichandran
  • Paul A. Roche
Original Articles

DOI: 10.1007/BF02736780

Cite this article as:
Ravichandran, V. & Roche, P.A. J Mol Neurosci (1997) 8: 159. doi:10.1007/BF02736780

Abstract

Syntaxins are transmembrane proteins that function in regulating transport vesicle docking and fusion with target membranes in neuronal and nonneuronal cells. Vesicle docking is thought to be regulated in part by the specific interactions of syntaxin with a vesicle-associated membrane protein termed synaptobrevin/VAMP. We have cloned a 1557-bp cDNA that encodes the human syntaxin 5 isoform, using a combination of PCR and colony-screening methods. The deduced 301 amino-acid sequence of human syntaxin 5 shares 96% identity with rat syntaxin 5. Like rat syntaxin 1A, human syntaxin 5 binds to synaptobrevin/VAMP in vitro. The identification of human syntaxin 5 as a synaptobrevin/VAMP-binding protein supports the hypothesis that syntaxin 5 regulates protein transport by binding to vesicle-associated membrane proteins.

Index Entries

SyntaxinsynaptobrevinSNAP-25vesicular transport

Copyright information

© Humana Press Inc 1997

Authors and Affiliations

  • Veerasamy Ravichandran
    • 1
  • Paul A. Roche
    • 1
  1. 1.Experimental Immunology Branch, National Cancer InstituteNational Institutes of HealthBethesda