, Volume 37, Issue 4, pp 265-292

Subcellular interactions between parallel fibre and climbing fibre signals in purkinje cells predict sensitivity of classical conditioning to interstimulus interval

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Classical conditioning of the nictitating membrane response requires a specific temporal interval between conditioned stimulus and unconditioned stimulus, and produces an incrase in Protein Kinase C (PKC) activation in Purkinje cells. To evaluate whether biochemical interactions within the Purkinje cell may explain the temporal sensitivity, a model of PKC activation byCa 2+, diacylglycerol (DAG), and arachidonic acid (AA) is developed.Ca 2+ elevation is due to CF stimulation and IP3 inducedCa 2+ release (IICR). DAG andIP 3 result from PF stimulation, while AA results from phospholipase A2 (PLA 2). Simulations predict increased PKC activation when PF stimulation precedes CF stimulation by 0.1 to 3 s. The sensitivity of IICR to the temporal relation between PF and CF stimulation, together with the buffering system of Purkinje cells, significantly contribute to the temporal sensitivity.