Effect of a nonmetabolizable analog of fructose-1,6-bisphosphate on glycolysis and ethanol production in strains ofSaccharomyces cerevisiae andEscherichia coli
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Fructose-1,6-bisphosphate (F-1,6-P2) is an allosteric activator of two key enzymes of glycolysis: phosphofructokinase and pyruvate kinase. Regulation of glycolysis in a wild-typeSaccharomyces cerevisiae and a recombinantEscherichia coli by a dead-end structural analog of F-1,6-P2 was studied. 2,5-Anhydromannitol (2,5-AM), a structural analog of β-d-fructose, was used. On being taken up by the cells, 2,5-AM was converted into its monophosphate and diphosphate by the enzymes of the glycolytic pathway. The final product, 2,5-anhydromannitol-1,6-bisphosphate, could not be metabolized further and, therefore, accumulated inside the cells. Glucose and fructose were used as substrates. It was found that 2,5-AM at concentrations of 1 mM or less did not have any effect on either substrate consumption or ethanol production. At concentrations of 2,5-AM of 2.5 mM or greater, significant inhibition of both glucose and fructose was observed, with fructose inhibition much more severe. We discuss the possible mechanisms of glycolysis inhibition by 2,5-AM at high concentrations and the regulation of glycolysis by this compound.
- Stryer, L. (1981), inBiochemistry, 2nd ed., Freeman, New York, pp. 266–268.
- Passonneau, J. V. and Lowry, O. H. (1963),Biochem. Biophys. Res. Commun. 13, 372–379. CrossRef
- Lad, P. M., Hill, D. E., and Hammes, G. G. (1973),Biochemistry 12, 4303–4309. CrossRef
- Hohmann, S. (1997), inYeast Sugar Metabolism, Zimmermann, Entian F. K. and Entian, K.-D., eds., Technomic, Lancaster, PA, pp. 171–186.
- Jurica, M. S., Mesecar, A., Heath, P. J., Shi, W., Nowak, T., and Stoddard, B. L. (1998),Structure 6, 195–210. CrossRef
- Davies, S. E. C. and Brindle, K. M. (1992),Biochemistry 31, 4729–4735. CrossRef
- Raushel, F. M. and Cleland, W. W. (1973),J. Biol. Chem. 248, 8174–8177.
- Marcus, C. J. (1976),J. Biol. Chem. 251, 2963–2966.
- Hartman, F. C. and Barker, R. (1965),Biochemistry 4, 1068–1075. CrossRef
- Frey, W. A., Fishbein, R., deMaine, M. M., and Benkovic, S. J. (1977),Biochemistry 16, 2479–2484. CrossRef
- Riquelme, P. T., Wernette, M. E., Kneer, N. M., and Lardy, H. A. (1983),Proc. Natl. Acad. Sci. USA 80, 4301–4305. CrossRef
- Luria, S. E. and Delbruck, M. (1943),Genetics 28, 491–511.
- Nghiem, N. P. and Davison, B. H. (2002), Oak Ridge National Laboratory Report Number ORNL/TM-2002/164, pp. 61–63.
- Koerner, T. A. W., Younathan, E. S., Ashour, A.-L., and Voll, R. J. (1974),J. Biol. Chem. 249, 5749–5754.
- Lin, E. C. C. (1987), inEscherichia coli and Salmonella typhimurium, vol. 1, Neidhardt, F. C., Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M., and Umbarger, H. E., eds., American Society for Microbiology, Washington, DC, pp. 262–264.
- Effect of a nonmetabolizable analog of fructose-1,6-bisphosphate on glycolysis and ethanol production in strains ofSaccharomyces cerevisiae andEscherichia coli
Applied Biochemistry and Biotechnology
Volume 141, Issue 2-3 , pp 335-347
- Cover Date
- Print ISSN
- Online ISSN
- Humana Press
- Additional Links
- Glycolysis regulation
- Saccharomyces cerevisiae
- Escherichia coli
- β-d-fructose structural analog
- fructose-1, 6-bisphosphate
- Industry Sectors