In Vitro Cellular & Developmental Biology - Animal

, Volume 32, Issue 10, pp 602–611

Human and animal mesenchymal progenitor cells from bone marrow: Identification of serum for optimal selection and proliferation

  • Donald P. Lennon
  • Stephen E. Haynesworth
  • Scott P. Bruder
  • Neelam Jaiswal
  • Arnold I. Caplan
Biotechnology

DOI: 10.1007/BF02724045

Cite this article as:
Lennon, D.P., Haynesworth, S.E., Bruder, S.P. et al. In Vitro Cell.Dev.Biol.-Animal (1996) 32: 602. doi:10.1007/BF02724045

Summary

An undifferentiated subset of cells within the stromal cell population of bone marrow in postnatal mammals retains the capacity to differentiate along osteogenic, adipogenic, fibroblastic, and chondrogenic lines. These cells, which are referred to as mesenchymal stem cells (MSCs), can be maintainedin vitro and expanded in number through a process of subculturing. MSCs are maintained in culture in medium supplemented with 10% fetal bovine serum (FBS). It is believed that certain, as yet unidentified, serum components play critical roles in the attachment and proliferation of MSCs. Commercially available FBS is poorly characterized and may vary in composition and quality from lot to lot. This study describes a method for the selection of lots of FBS that best support maintenance of the undifferentiated state, mitotic expansion of MSCsin vitro, and retention of multilineage developmental potential in response to appropriate cues.

Key words

fetal bovine serum proliferation mesenchymal stem cell marrow osteochondral potential 

Copyright information

© Society for In Vitro Biology 1996

Authors and Affiliations

  • Donald P. Lennon
    • 1
  • Stephen E. Haynesworth
    • 1
  • Scott P. Bruder
    • 2
    • 3
  • Neelam Jaiswal
    • 3
  • Arnold I. Caplan
    • 1
  1. 1.Skeletal Research Center, Department of BiologyCase Western Reserve UniversityCleveland
  2. 2.Department of OrthopaedicsCase Western Reserve UniversityCleveland
  3. 3.Osiris Terapeutics, Inc.Baltimore