Journal of Biosciences

, Volume 14, Issue 3, pp 279–287

Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay

  • Kakoli Banerjee
  • Gita Sharma
  • S. Upadhyay
  • B. S. Anand
  • G. S. Raju
  • P. S. Khandekar
Article

DOI: 10.1007/BF02716689

Cite this article as:
Banerjee, K., Sharma, G., Upadhyay, S. et al. J Biosci (1989) 14: 279. doi:10.1007/BF02716689

Abstract

A DNA hybridization assay was developed using a cloned hepatitis B viral genome to detect the presence of infectious virions in human serum. The merit of this assay was to put in evidence virus particles in 7 out of 133 sera that were negative for surface antigen (HBsAg) using routine serological methods. The usefulness of this assay was confirmed by actual visualization of the virus under electron microscope. Some serum samples although positive for surface antigen, did not give a hybridization signal by dot blot assay and might indicate cases of acute hepatitis

Keywords

DNA dot hybridization hepatitis B virus integration 

Abbreviations used

HBV

Hepatitis B virus

RPHA

reverse passive hacmaglutination assay

SDS

sodium dodecyl sulphate

SSC

sodium citrate saline

Copyright information

© Indian Academy of Sciences 1989

Authors and Affiliations

  • Kakoli Banerjee
    • 1
  • Gita Sharma
    • 1
  • S. Upadhyay
    • 1
  • B. S. Anand
    • 1
    • 2
  • G. S. Raju
    • 1
    • 2
  • P. S. Khandekar
    • 1
  1. 1.Gene Expression LaboratoryNational Institute of ImmunologyNew DelhiIndia
  2. 2.Department of GasteroenterologyG. B. Pant HospitalNew DelhiIndia