Journal of Biosciences

, Volume 10, Issue 1, pp 145–151

Mechanism of action of aflatoxin B1

  • R. P. Tiwari
  • T. C. Bhalla
  • S. S. Saini
  • G. Singh
  • D. V. Vadehra
Article

DOI: 10.1007/BF02702849

Cite this article as:
Tiwari, R.P., Bhalla, T.C., Saini, S.S. et al. J Biosci (1986) 10: 145. doi:10.1007/BF02702849

Abstract

The inhibitory effects of aflatoxin B1 were found to be related to the gram character in procaryotes, used in this study. Ethylene diamine tetra chloroacetic acid (0.05% w/v) or Tween-80 (0.05 % v/v) addition accentuated the aflatoxin B1 growth inhibition inSalmonella typhi andEscherichia coli at different pH values. The inhibition of lipase production was only 5–20 % inPseudomonas fluorescence ca. 25–48% inStaphylococcus aureus andBacillus cereus at different aflatoxin B1 concentrations (4–16μg/ml).However, inhibition of α-amylase induction was complete in1Bacillus megaterium whereas the inhibition was partial inPseudomonas fluorescence (27–40%) at 32μg aflatoxin B1 concentration. An increase in leakage of cell contents and decreased inulin uptake were observed in toxin incubated sheep red blood cell suspension (1 %) with increased aflatoxin B1 concentration

Keywords

Aflatoxin B1α-amylasecell content leakageenzyme induction/inhibitionlipase procaryotes

Abbreviations used

EDTA

Ethylene diamine tetra chloroacetic acid

SRBC

sheep red blood cell

Copyright information

© Indian Academy of Sciences 1986

Authors and Affiliations

  • R. P. Tiwari
    • 1
  • T. C. Bhalla
    • 1
  • S. S. Saini
    • 1
  • G. Singh
    • 1
  • D. V. Vadehra
    • 1
  1. 1.Department of MicrobiologyPanjab UniversityChandigarhIndia