Journal of Biosciences

, Volume 4, Issue 3, pp 257–261

α-D-galactose-specific lectin from jack fruit (A rtocarpus integra) seed

  • G. Suresh Kumar
  • P. S. Appukuttan
  • Debkumar Basu
Article

DOI: 10.1007/BF02702736

Cite this article as:
Suresh Kumar, G., Appukuttan, P.S. & Basu, D. J Biosci (1982) 4: 257. doi:10.1007/BF02702736

Abstract

An α-D-galactose-specific lectin from the seeds of jack fruit (Artocarpus integra) has been isolated in pure form by affinity chromatography on immobilised guar gum (a galactomannan). The lectin is shown to be a glycoprotein containing 3% carbohydrate and having a molecular weight of 39,500 as determined by gel filtration. Sodium dodecyl sulphate gel electrophoresis revealed a single polypeptide of 10,500 dalton, indicating that the native lectin is a tetrarner of identical subunits. The hemagglutinating activity of the lectin towards erythrocytes of all blood groups is found to be the same.

Keywords

Jack fruit seedlectinα-D-galactoseguar gumaffinity chromatography

Abbreviations used

Con A

Concanavalin A

PBS

20 mM potassium phosphate buffer pH 7.4 containing 150 mM NaCl

Copyright information

© Indian Academy of Sciences 1982

Authors and Affiliations

  • G. Suresh Kumar
    • 1
  • P. S. Appukuttan
    • 1
  • Debkumar Basu
    • 1
  1. 1.Neurochemistry DivisionSree ChitraTirunal Institute for Medical Sciences and TechnologyTrivandrum