α-D-galactose-specific lectin from jack fruit (A rtocarpus integra) seed
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- Suresh Kumar, G., Appukuttan, P.S. & Basu, D. J Biosci (1982) 4: 257. doi:10.1007/BF02702736
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An α-D-galactose-specific lectin from the seeds of jack fruit (Artocarpus integra) has been isolated in pure form by affinity chromatography on immobilised guar gum (a galactomannan). The lectin is shown to be a glycoprotein containing 3% carbohydrate and having a molecular weight of 39,500 as determined by gel filtration. Sodium dodecyl sulphate gel electrophoresis revealed a single polypeptide of 10,500 dalton, indicating that the native lectin is a tetrarner of identical subunits. The hemagglutinating activity of the lectin towards erythrocytes of all blood groups is found to be the same.
KeywordsJack fruit seedlectinα-D-galactoseguar gumaffinity chromatography
- Con A
20 mM potassium phosphate buffer pH 7.4 containing 150 mM NaCl